Proudly sponsored by:
Noble Foundation
Pennington Seed
AGresearch
PGG Wrightson Seed
Grasslanz
NZ Grassland Trust
Agrinostics
6th International Symposium on Fungal Endophytes of Grasses

Programme

The symposium will have a mix of social events, five scientific sessions and a field trip.

The symposium will open on the Sunday evening (25 March) and close late on Wednesday afternoon (28 March).

Full Programme

Sunday – 25 March

3.00 - 6.00pm Registration
6.00 - 8.30pm Welcome and opening

Monday – 26 March am

7.30 - 8.30am Registration

Session 1: Fungal Endophytes – The Influential Symbiosis
Chairman: Adrian Leuchtmann
Sponsored by:

8.30 - 9.00am The importance of endophyte in agricultural systems - changing plant and animal productivity
Syd Easton, AgResearch, New Zealand.
9.00 - 9.30am Community and ecosystem consequences of endophyte symbiosis with tall fescue
Keith Clay & Jennifer Rudgers, Indiana University and Rice University, USA
9.30 - 10.00am Variability is the nature of the endophyte-grass interaction
S.H. Faeth and K. Saikkonen, Arizona State University, Arizona, USA and MTT Agrifood Research, Finland
10.00 - 10.15am High Neotyphodium infection frequencies in tillers and seed of infected wild fescue plants
Steve L. Clement, L.R. Elberson, and M. Kynaston USDA-ARS, Washington State University, Pullman, Washington, U.S.
10.15 - 10.30am Fungal grass endophytes, grass cultivars, nitrogen deposition and the associations with colonising insects
Jochen Krauss, Simone A. Härri, Lowell Bush, Sally Power and Christine B. Müller, University of Zürich, Switzerland
10.30 - 10.45am Using a multidisciplinary approach with undergraduate students to study the grass endophyte system
T.L. Bultman, T.J. Sullivan, D. Swarthout, K. Brown and T. Pennings, Hope College, Holland, MI USA
10.45 - 11.45am Tea and Poster Viewing
11.45 – 12.00pm Poster Summary
Poster facilitator: Charles Bacon
12.00 - 12.30pm Poster Discussion
12.30 - 1.30pm LUNCH

Monday – 26 March pm

Session 2: Mechanics of the symbiosis
Chairman: Nick Hill
Sponsored by:
1.30 – 2.00pm Plant influences on endophyte expression
C.P. West, University of Arkansas, Fayetteville, AR, USA
2.00 – 2.30pm The biology of the endophyte/grass partnership
M.J Christensen and C.R. Voisey, AgResearch Grasslands, Palmerston Nth, New Zealand
2.30 – 2.45pm High nutrient supply and carbohydrate content reduce endophyte and alkaloid concentration
Susanne Rasmussen, Anthony J. Parsons, Q. Liu, Hong Xue and Jonathan A. Newman, AgResearch Grasslands, Palmerston North, New Zealand
2.45 – 3.00pm Distribution and morphology of Neotyphodium endophytes in apical meristems of host plants as observed using differential interface contrast microscopy
Koya Sugawara, Hiroto Ohkubo and Takao Tsukiboshi, National Institute of Livestock and Grassland Science, Nasushiobara, Japan
3.00 – 3.15pm Exploring endophyte compatibility in perennial ryegrass
M.J. Faville, A. Koulman, L. Briggs, M. Cao, C. Podmore, and D. Abraham, AgResearch, New Zealand
3.15 – 3.30pm Inducible defenses provided by Neotyphodium to Lolium arundinaceum and Lolium pratense: an ecological and molecular approach
T. J. Sullivan, T. L. Bultman, J. Rodstrom, J. Vandop, J. Librizzi, C. Graham, A. Sielaff and L. Fernandez, Hope College, Holland, MI USA
3.30 – 4.30pm Tea and Poster Viewing
4.30 – 4.45pm Poster Summary
Poster Facilitator: Brian Tapper
4.45 – 5.15pm Poster Discussion
Evening FREE

Tuesday – 27 March am

Session 3 Application of Endophyte Technologies
Chairman: Tony Stratton
Sponsored by:
8.00 - 8.30am Delivering quality seed to specification
Phil Rolston and Chris Agee, AgResearch, Lincoln, New Zealand and Pennington Seeds, Madison, USA
8.30 - 9.15am

Technology transfer – successes and problems.

  • Transferring endophyte technology to North American farmers
    J.G. Andrae and C.A. Roberts, Clemson University, and University of Missouri, USA
  • Technology transfer of novel ryegrass endophytes in New Zealand
    G.D. Milne, Agricom Ltd, Christchurch, New Zealand
  • Commercialisation of AR1 in Australia  
    J.W. Evans, Heritage Seeds, Victoria, Australia
9.15 - 9.45am On-farm impacts of new technologies
Jane Parrish, Richard Watson, Lester Fletcher, Mississippi State University, USA and AgResearch, New Zealand
9.45 - 10.15am Tea and Posters
10.15 - 10.30am Poster Summary
Poster facilitator: Craig Roberts
10.30 - 10.50am Poster Discussion

Tuesday – 27 March pm

11.00 - 5.30pm Field Day (followed by drinks)
6.00pm CONFERENCE DINNER
Sponsored by:

Wednesday - 28 March am

Session 4 Endophyte, plant, herbivore interactions
Chairman: Steve Clement
Sponsored by:


8.00 - 8.30am Anti-herbivore factors of grass endophytes: New prospects from metabolomics 
G.A. Lane, M. Cao, L.J. Johnson, A. Koulman, A.J. Popay, S. Rasmussen and B.A. Tapper, AgResearch, New Zealand
8.30 - 9.00am Mediation of herbivore-natural enemy interactions by Neoptyphodium endophytes:  The role of insect behavioural response
D S. Richmond, Purdue University, USA
9.00 - 9.15am Grass cultivar diversity and endophyte infection affect abundance of herbivores and their natural enemies
C.B. Müller, S.A, Härri, V. Boreux and J. Krauss, University of Zürich, Switzerland
9.15 - 9.30am Endophyte-mediated multitrophic interactions in Lolium pratense
P. Lehtonen, M. Helander and K. Saikkonen, MTT Agrifood Research Finland, Finland
9.30 - 9.45am Mode of action of tremorgens
Sarah Finch, AgResearch, New Zealand
9.45 - 10.00am Effect of perennial ryegrass endophyte and a feed additive on some physiological parameters and intake of young ewes in winter
M L Henry, M Hazelton, K F M Reed, L J Cummins, B J Leury, University of Melbourne, Australia
10.00 - 10.15am Clinical manifestations of tall fescue (Festuca arundinacea) and perennial ryegrass (Lolium perenne) toxicosis in Oregon and Japan
Linda L. Blythe, A. Morrie Craig, Charles Estill and Chris Cebra, Oregon State University, USA
10.15 - 11.15am Tea and posters
11.15 - 11.30am Poster Summary 4A Invertebrates
Poster Facilitator: Alison Popay
11.30 - 11.45am Poster Discussion 4A Invertebrates
11.45 - 12.00pm Poster Summary 4B Animals
Poster Facilitator: Lester Fletcher
12.00 - 12.15pm Poster Discussion Animals 
12.15 - 1.15pm LUNCH

Wednesday - 28 March pm

Session 5 The diversity of endophytes
Chairman: Suzanne Rasmussen
Sponsored by:
1.15 - 1.45pm Functional analysis of the Epichloë festucae-perennial ryegrass symbiosis
B. Scott, D. Takemoto, A. Tanaka, C.A. Young, M.K. Bryant and K.J. May, Massey University, Palmerston Nth, New Zealand
1.45 - 2.05pm Functional genomics of the Neotyphodium lolii / ryegrass symbiosis
R.D. Johnson, S. Bassett, M. Christensen, C. Gaborit, L. Johnson, A. Khan, A. Koulman, S. Rasmussen, C. Voisey and G. Bryan, AgResearch Grasslands, Palmerston Nth, New Zealand
2.05 - 2.35pm Relationships of Epichloë typhina isolates from different host grasses
C.L. Schardl, A. Leuchtmann, and B.A. McDonald, University of Kentucky, USA
2.35 - 2.50pm The role of cAMP signalling in the symbiosis between Epichloë festucae and Lolium perenne
C.R. Voisey, M.J. Christensen, R.D. Johnson, L.J. Johnson, A.K. Khan, Z.A. Park, M. Ramakrishna, M. Cao, J.P. Koolaard, A. Koulman, S. Borchert, C.N. Gaborit, J.M. Pratt, S. Rasmussen, G.T. Bryan, AgResearch Grasslands, Palmerston Nth, New Zealand
2.50 - 3.05pm A gene cluster for ergovaline biosynthesis
D. J. Fleetwood, B. Scott, G. A. Lane, A. Tanaka and R. D. Johnson AgResearch, Palmerston Nth, New Zealand
3.05 - 3.45pm Tea and posters
3.45 - 4.00pm Poster Summary
Poster Facilitator: Christine Voisey
4.00 - 4.20pm Poster Discussion
4.20 - 4.50pm Final Speaker
Where Do We Go From Here?
Joe Bouton, The Samuel Roberts Noble Foundation, Ardmore, USA
4.50 - 5.00pm CONFERENCE CLOSE

Posters

Poster Session 1

Role of endophytic fungi in grass litter decomposition…. Takashi Osono, KyotoUniversity, Kyoto, Japan

The endophytic community of Dactylis glomerataSalud Sánchez Márquez, Gerald F. Bills, Íñigo Zabalgogeazcoa, Instituto de Recursos Naturales y Agrobiologia,Salamanca, Spain

Influence of toxic endophyte-infected tall fescue on the prevalence of E. coli O157:H7 from ruminants, a review….M.L. Looper, T.S. Edrington, T.R. Callaway, R. Flores, G.E. Aiken, J.M. Burke and C. F. Rosenkrans Jr, USDA-ARS, Booneville, USA

Botanophila flies on Epichloë host species in Europe and North America: no evidence for coevolution….Adrian Leuchtmann, ETH Zürich,Switzerland

The distribution and incidence of Neotyphodium endophytes in native grasses from Argentina and their association with environmental factors…..D. Cabral, L.J. Iannone, A. Stewart and M.V. Novas, Universidad de Buenos Aires, Buenos Aires, Argentina.

Distribution of Neotyphodium endophytes in naturalised ryegrasses (Lolium spp) in Japan….M. Yamshita, K. Uchiyama, T. Matsuda, H. Tobina, H. Sawada, K. Sugawara, ShizuokaUniversity, Shizuoka, Japan

‘Staggers’ in Australian alpaca (Vicugna pacos)…. Natalia Sampaio, Mark Gishen, K.F.M. Reed, Mel Brown, Daphne Gregory and Kylie Munyard, Curtin University of Technology, Perth, Australia

Neotyphodium spp.–grass association in natural grassland in Argentina…. M.N.  Colabelli, M.S. Torres, A.M. Clausen, O.N. Fernandez and A. Peretti, Universidad Nacional de Mar del Plata, Balcarce, Argentina

Were endophytes pre-adapted for defensive mutualism? ... M.S. Torres, A.P. Singh, N. Vorsa, T. Glanfagna and J.F.White Jr., Rutgers University, New Jersey, USA

Incidence and diversity of Neotyphodium endophytes in Bulgarian populations of Lolium perenne …. H.Hahn, K. Andreeva and E. Willner, Martin-Luther-University Halle-Wittenberg, Germany, University of Kentucky, USA and IPK, Germany

Neotyphodium impacts on soil nematode communities: rhizosphere and litter-mediated interactions…. M. Omacini, E.J. Chaneton and C.M. Ghersa, Universidad de Buenos Aires, Argentina

Disease and pest resistance in endophyte infected and non-infected drunken horse grass….Chunjie Li, Xingxu Zhang, Fei Li, Zhibiao Nan and Christopher Schardl, LanzhouUniversity, Lanzhou, China and University of Kentucky, USA

Occurrence and genetic structure of Epichloë festucae endophytes in wild red fescue populations and their seed progenies…. K. Saikkonen, P. Wali and M. Helander, MTT Agrifood Research Finland, Finland

Endophytic fungi from Catharanthus roseus (L.) G. Don: A preliminary study… R.N. Kharwar, BanarasHinduUniversity, Varanasi, India

Occurrence of Neotyphodium endophytes of perennial ryegrass (Lolium perenne) and fescue grass (Festuca spp.), and characterisation of selected isolates with respect to toxin production, drought and insect resistance….Prathap Kumar Halady Shetty, Anne Mette Dahl Jensen, Niels Roulund and Birte Boelt, Danish Institute of Agricultural Sciences, Denmark

Poster Session 2

Systems level modelling of metabolism in Neotyphodium lolii endophyte- implications for symbiosis with ryegrass…. Rajiv Chaturvedi, Tanya Soboleva, Anthony J. Parsons and Susanne Rasmussen, AgResearch, New Zealand

Differential expression of loline alkaloids in perennial ryegrass infected with endophyte isolated from tall fescue…. H.S. Easton, T.B. Lyons, W. Mace, W.R. Simpson, A. de Bonth, B.M. Cooper, K. Pankhurst  AgResearch,  New Zealand

Degradation of endophyte alkaloids in field-dried cut ryegrass herbage…. D.E. Hume, M.J. Hickey, B.A. Tapper and L.R. Fletcher, AgResearch, New Zealand

Structure elucidation of 10,11-epoxyjanthitrem B and isolation of janthitrem B from Penicillium janthinellum cultures…. J.V. Babu, A.L. Wilkins, C.O. Miles and S.C. Finch, AgResearch, Hamilton, New Zealand

Conspicuous epiphytic growth of a Neotyphodium endophyte on perennial ryegrass inflorescences…. M.J. Christensen and Keith Salisbury, AgResearch and PGG Wrightson, New Zealand

Biosynthesis of an extracellular siderophore is essential for maintenance of mutualistic endophyte-grass symbioses…..L. J. Johnson, M. Steringa, A. Koulman, M. Christensen, R. D. Johnson, C. R. Voisey, G. Bryan, I. Lamont and S. Rasmussen, AgResearch, Palmerston North, New Zealand

The analysis of fungal alkaloids in plant fluids…. Albert Koulman, Marit van Gils, Geoffrey A. Lane, Mike J. Christensen, Karl Fraser and Brian A. Tapper, AgResearch, Palmerston North, New Zealand

Ménage à trois– are two fungi too much for ryegrass?…Qianhe Liu, Anthony J. Parsons, Hong Xue, Heike Harze, and Susanne Rasmussen, AgResearch Ltd., Palmerston North, New Zealand

Endophyte production of reactive oxygen species is critical for maintaining the mutualistic symbiotic interaction between Epichloë festucae and Pooid grasses…. A. Tanaka, M.J. Christensen,  D. Takemoto and B. Scott, Massey University, Palmerston Nth, New Zealand

Metabolic profiling of endophyte-infected and endophyte-free ryegrass grown under sufficient water supply and drought….H. Hahn, O. Fiehn, M.A. McManus and D.B. Scott, Martin-Luther-University Halle-Wittenberg, Germany, UC Davis Genome Center, USA and Massey University, Palmerston Nth.

Isolation of symbiotic mutants of Epichloë festucae using Agrobacterium mediated T-DNA mutagenesis….A. Tanaka, D. Takemoto, R. Wrenn and B. Scott, Massey University, Palmerston Nth, New Zealand

Discovery of small molecule bioactive agents from endophytic fungi of the Sonoran Desert….B.P. Bashya, A.M. Burns, M. X. Liu, P.A. Paranagama, C.R J. Seliga, T.J. Turbyville, E.M. Kithsiri Wijeratne, J. Zhan, M.K. Gunatilaka, A.E. Arnold, S.H. Faeth, L. Whitesell, and A.A.L. Gunatilaka, University of Arizona, Tempe, Arizona USA

Neotyphodium hybrid identity determines alkaloid production, herbivory, and plant biomass production in two native grasses….Cyd Hamilton, Stan Faeth, Andrea Jani, Cinnamon Hayes, Arizona State University, USA

Endophyte survival without sex….B.R. Holland, C.L. Schardl and J. Schmid, Massey University, Palmerston Nth, New Zealand and University of Kentucky, USA

Dehydrin expression in drought-stressed tall fescue….C. A. Guerber, C. P. West, R. D. Carson, A.M. Havely, University of Arkansas, Fayetteville, AR USA

Neotyphodium lolii induces a limited host defence response by Lolium perenne….Ningxin Zhang, Mark Raftery, Kim Richardson, Michael J. Christensen and Jan Schmid, Massey University and AgResearch, Palmerston Nth, New Zealand

Neotyphodium interactions with a native grass are driven by endophyte haplotype….Laura J. Morse, Stanley H. Faeth and Thomas A. Day, ArizonaStateUniversity, Tempe, Arizona

Spontaneous in planta changes in fungal endophytes impact symbiosis….Wayne Simpson, AgResearch Grasslands, Palmerston Nth, New Zealand

Poster Session 3

Introduction and acceptance of novel endophyte tall fescue in the USA….D.M. Ball, G.D. Lacefield, C.S. Agee, and C.S. Hoveland, Auburn University, USA

The effect of the Max P endophyte in tall fescue on pasture production and composition, and sheep grazing preference, in a dryland environment….K.N. Tozer, S. Ates, N.R. Mapp, M.C. Smith, R.J. Lucas and G.R. Edwards, Lincoln University, Canterbury, New Zealand

Germination rate and seedling vigour of tall fescue as affected by endophyte status and seed density….H. Troye Owens, C.H. Finneseth, T. M. Tillery and T.D. Phillips, University of Kentucky, USA

A new generation ryegrass endophyte – the first results from dairy cows fed AR37… E.R. Thom, C.D. Waugh, E.M.K. Minnee and G.C. Waghorn, Dexcel Ltd, Hamilton, New Zealand

Advantages of endophyte infection for irrigated pastures of semiarid, cold-desert environments…Blair L. Waldron, USDA-ARS, Logan, Utah, USA

A new tall fescue – novel endophyte combination for the south central USA. A.A. Hopkins and J. H. Bouton, The Samuel Roberts Noble Foundation, Ardmore, USA 

Improving sustainability and quality of ryegrass production in Wairarapa, New Zealand…. M.R. McNeill, D.E. Hume, R. Ashby, M. Ashby, P. Brandon, P. Guscott, B. Tosswill and R. Barton, AgResearch, New Zealand 

Differing agronomic responses of Italian/hybrid ryegrasses to infection with Neotyphodium loli….B. M. Cooper, D. E. Hume, K. A. Panckhurst and, A. J. Popay, AgResearch, New Zealand 

Comparison of ergovaline determinations between the laboratories in the United States and Japan…..A. Morrie Craig, G. Rottinghaus, K. Walker and E. Ishikuro, Oregon State University, Corvallis, OR USA

Animal performance, carcass quality and economics of cattle finished after grazing endophyte-infected, endophyte-free or nonergot alkaloid-producing endophyte-infected tall fescue…..S. K. Duckett, R.C. Lacy, J.G. Andrae, C.S. Hoveland, J.H. Bouton and M. A. McCann, Clemson University, Clemson, USA 

Endophyte fungus levels in perennial ryegrass and tall fescue varieties grown for seed in Oregon, USA – Implications for responsible grass straw use as livestock feed. W.C. Young III, M.E. Mellbye, T.B. Silberstein, G.A. Gingrich, A.M. Craig and L.L. Blythe, Oregon State University, Corvallis, USA 

Endophyte survival during seed storage.   Nicholas Hill, Agrinostics  Ltd. Co., GA, USA

Effects of Neotyphodium endophyte infection on seed viability and germination vigour in Lolium multiflorum under accelerated ageing conditions…. P.E. Gundel, J.B. Landesmann, M.A. Martinez-Ghersa and C.M. Ghersa, Universidad de Buenos Aires, Buenos Aires, Argentina 

AR1 Quality Assurance by ELISA….L.R. Briggs, J.M. Sprosen, B.A. Tapper and H.S. Easton, AgResearch, Hamilton, New Zealand

Tiller survival after drought of 'Grasslands Flecha' tall fescue as influenced by endophyte…. C.P. West, F. Volaire, and F. Lelievre, University of Arkansas, Fayetteville, AR, USA

Poster Session 4A

Can aphids learn to cope with the presence of endophytic fungi in their food plants?.... S. A. Härri, J. Krauss and C.B, Müller, University of Zürich, Switzerland 

Variable performance of bird cherry-oat aphid on I – infected wild tall fescue from Tunisia….. Steve L. Clement, L.R. Elberson, B.L. Waldron, and S.S. Quisenberry, USDA- ARS, Washington State University, Pullman, WA USA

Cereal leaf beetle: Is performance affected by grass endophytes?....Steve L. Clement, L.R. Elberson, T. Miller, M. Kynaston and T. Phillips, USDA, ARS, WashingtonStateUniversity, Pullman,WAUSA

Endophytic fungi as indirect defense mechanism in grasses - mycotoxins enhance the UV-visibility of herbivore urine in the eyes of predators…..Susanna Saari, Otso Huitu, Marjo Helander, Päivi Lehtonen and Kari Saikkonen, Agricultural Research Centre of Finland, Finland

Reductions in root aphid populations by three non-toxic endophyte strains in tall fescue. Joanne Jensen and Alison Popay, AgResearch, Hamilton, New Zealand

Influence of genetic variation in fungal endophyte on the third trophic level….. J.L. Rodstrom, T. Bultman, J. VanDop, J. Librizzi, L. Longwell, and T. Sullivan. HopeCollege, Holland, MI, USA

The effect of endophyte in perennial ryegrass and tall fescue on red and blackheaded  cockchafers….B.M. Watson, Toora, Victoria, Australia

Endophyte reduces mealybug infestation in tall fescue in Australia….A.J. Popay, D.E. Hume and K. Low, AgResearch, New Zealand

Endophyte effects on consumption of seed and germinated seedlings of ryegrass and fescue by grass grub (Costelytra zealandica) larvae….A.J. Popay and B.A. Tapper, AgResearch, New Zealand

Nematotoxic effects of endophyte-infected tall fescue toxins and extracts to an in vitro bioassay using the nematode Pratylenchus scribneri.A.A. Bacetty, M.E. Snook, A.E. Glenn, C.W. Bacon, C.L. Schardl and P. Nagabhyru, University of Kentucky, Lexington, USA

Poster Session 4B

Influence of endophyte-infected fescue on serum concentrations of leptin in female ruminants…. J.M. Burke, D.H. Keisle and, R.W. Rorie, U.S. Department of Agriculture, Booneville, USA 

Toxicological evaluation of endophyte-infected perennial ryegrass straw to Japanese Black steers….S. Miyazaki, M. Hanazumi, T. Ikeda, Y. Fukumoto, T. Yamata, O. Mikami, N. Yamanaka, H. Murata and N. Shimada, NARO, Ibaraki, Japan

Epidemiological studies with the mare and N. coenophialum toxins….F. F. Fannin, Danica Kubly, Yan Wang and Lowell Bush, University of Kentucky, Lexington, USA

Endophyte toxin residues in animal tissue…. Sarah Finch, AgResearch, Hamilton, New Zealand

Determination of the toxic threshold of lolitrem B in cattle eating endophyte-infected perennial ryegrass….. Linda Blythe, Charles Estill, James Males and A. Morrie Craig, OregonStateUniversity, Corvallis, OR, USA

A review of the ergot alkaloids found in endophyte-infected tall fescue and perennial ryegrass and their metabolism after ingestion by livestock….. Jennifer M. Duringer, Marthah J.M. DeLorme, Andreas Lehner and A. Morrie Craig, Oregon State University, Corvallis, USA

Discovery of novel microorganisms involved in ergot alkaloid detoxification: an approach….. S. Perumbakkam, R.M. Rattray, M.J.M. DeLorme, J.M. Duringer, and A.M. Craig, Oregon State University, Corvallis, USA

Responses to orally ingested Neotyphodium endophyte toxins in laboratory animals…. Tatsushi Inoue, Miyagi University, Japan

The influence of temperature variation upon vascular dynamics in cattle as measured by Doppler-image ultrasonography…..B.H. Kirch, G.E. Aiken, and D.E Spiers, USDA-ARS, Lexington, U.S.A

Development of a feed-intake model for evaluation of toxins associated with endophyte-infected fescue…. P. A. Eichen, S. L. Raney, G. E. Rottinghaus and D. E. Spiers, University of Missouri, USA

Development of a bovine model to evaluate administration routines for endophyte-infected tall fescue….L.E. Wax, G.E. Rottinghaus, and D.E. Spiers, University of Missouri, MI, USA

Individual and combinatorial assessment of vasoconstrictive capacity of tall fescue alkaloids using fescue-naïve bovine lateral saphenous veins….J.L. Klotz, B.H. Kirch, G.E. Aiken, L.P. Bush, B.C. Arrington and J.R. Strickland, USDA-ARS, Lexington, USA

Dynamics of peripheral bloodflow for endophyte-naïve cattle fed toxic tall fescue seed….  G.E. Aiken, B.H. Kirch, and J.R. Strickland, USDA-ARS, University of Kentucky, Lexington, USA

Body weight gain and voluntary feed intake in meat goat does fed endophyte-infected and endophyte-free tall fescue seed….R. Browning, Jr., B. Donnelly, T. Payton, P. Pandya and M. Byars, TennesseeStateUniversity, Nashville, USA.

Progress in breeding sheep for resistance to ryegrass staggers… C.A. Morris and N.C. Amyes, AgResearch, Hamilton, New Zealand

Poster Session 5

Analysis of genetic markers and ergovaline production in Neotyphodium endophyte-infected fescue species collected from Italy, Spain and Denmark….Anne Mette Dahl Jensen, Lisbeth Mikkelsen, Prathap Kumar Halady Shetty and Niels Roulund, Danish Institute of Agricultural Sciences, Denmark 

Joint analyses of transcriptomic and metabolomic data to probe the ryegrass-endophyte symbiosis….M. Cao, L. Johnson, R. Johnson, A. Koulman, G.A. Lane and S. Rasmussen, AgResearch Ltd., Palmerston North, New Zealand

High throughput direct infusion ion trap mass spectrometry: a new method for the chemotyping of endophytes…Albert Koulman, AgResearch, Palmerston North, New Zealand

Towards the elimination of ergot alkaloid biosynthesis genes in Neotyphodium coenophialum….S. Florea, C. Machado, D. Zhang, D.G. Panaccione and C.L. Schardl, University of Kentucky, Lexington, USA

Spatial and temporal expression patterns of lolitrem biosynthetic genes in the Epichloë festucae-perennial ryegrass symbiosis….K.J. May, M.K. Bryant, X. Zhang, B. Ambrose and B. Scott, Massey University, Palmerston Nth, New Zealand

Characterisation of fungal endophytes present in Elymus canadensis (Canada wild rye)…. Kirsti Burr, Shipra Mittal, Andy Hopkins and Carolyn Young, The Samuel Roberts Noble Foundation, US

Peramine and indole-diterpene biosynthetic capability of epichloë endophytes as predicted by perA and ltm gene analysis ….. B. Scott, C.A. Young, B.A. Tapper, R.E. Wrenn, S.J. Foster, C.D. Moon, C.L. Schardl, Massey University, Palmerston Nth, New Zealand

Identification of NRPS gene families from Neotyphodium/Epichloë endophytic fungi that form mutualistic associations with cool season grasses…. Richard Johnson, Christine Voisey, Jennifer Pratt, Linda Johnson and Gregory Bryan, AgResearch Grasslands, Palmerston Nth, New Zealand

Analysis of expressed sequence tags derived from the endophytic fungus Neotyphodium lolii grown in vitro and in association with its host plant perennial ryegrass….Richard Johnson, Anar Khan, Christine Voisey,  Shalome Bassett, Charlotte Gaborit, Linda Johnson, Mike Christensen, Alan McCulloch and Gregory Bryan, AgResearch Grasslands, Palmerston Nth, New Zealand

Targeted introduction of point mutations in Epichloë festucae…. Kurtis Knapp, Richard Johnson, Christine Voisey and Gregory Bryan, AgResearch Grasslands, Palmerston Nth, New Zealand

A gene identified from Neotyphodium lolii is expressed only in planta and regulates the biosynthesis of a putative oligopeptide secondary metabolite….Richard D Johnson, Sophie Borchert, Mike Christensen, Linda J Johnson, Albert Koulman, Marit J van Gils and Gregory Bryan, AgResearch Grasslands, Palmerston Nth, New Zealand

Dual Affymetrix gene-chip analysis of the perennial ryegrass-endophyte symbiosis…..L.J. Johnson, C.R. Voisey, R.D. Johnson, A.K. Khan, Z.A. Park, M. Ramakrishna, M. Cao, D. Simon, M. Christensen, G.T. Bryan and S. Rasmussen, AgResearch Grasslands, Palmerston Nth, New Zealand

The genome and the genes of Epichloe festucae…. Uljana Hesse, Bruce A. Roe, Jerzy W. Jaromczyk, Mark L. Farman, Kalina Andreeva, Love Gill, Paul Maynard, Venu-Gopal Puram and Christopher L. Schardl, University of Kentucky, Lexington, USA

Endophytes of the tall fescue ploidy series in Europe, North Africa and the Mediterranean… C.L. Schardl, K.D. Craven, K.K. Schweri, W. Hollin, S.L. Clement, J. Schmid, C.P. West, and T.D. Phillips, University of Kentucky, USA

Biosynthesis of insecticidal loline alkaloids by epichloë endophytes of grasses….Christopher L. Schardl, Robert B. Grossman, Jimmy D. Blankenship, Martin J. Spiering, and Jerome R. Faulkner, University of Kentucky, Lexington, USA

Microsynteny between Neotyphodium lolii and Fusarium graminearum.... Charlotte Gaborit, Anar Khan, Jennifer Pratt, Richard Johnson, Linda Johnson and Gregory Bryan, AgResearch Grasslands, Palmerston Nth, New Zeland

Heterologous synthesis of the fungal alkaloid peramine…. Steven D. Pirlo, Richard D. Johnson, Christine R. Voisey, Albert Koulman and Gregory T. Bryan, AgResearch Grasslands, Palmerston Nth, New Zealand

Development of an Affymetrix dual genome (Neotyphodium lolii/Lolium perenne) symbiosis genechip…. C. R. Voisey, A. K. Khan, Z A. Park, L. J. Johnson, R. D. Johnson, M. Ramakrishna, M. Cao, S Bassett, C.N. Gaborit,  A F McCulloch, D. Simon, S. Rasmussen, M. Christensen and G. T. Bryan, AgResearch Grasslands, Palmerston Nth, New Zealand

Characterisation of a novel endophyte NRPS gene and its role in endophyte-grass symbioses… Heike Harzer, Richard Johnson, Susanne Rasmussen, Christine Voisey and Linda Johnson, AgResearch Grasslands, Palmerston Nth, New Zealand

Double-stranded RNA viruses infecting Epichloë festucae….María Romoa, Adrian Leuchtmann, Balbino Garcíaa, Íñigo Zabalgogeazcoaa, Instituto de Recursos Naturales y Agrobiologia, CSIC, Salamanca, Spain